SIRT1 Protects Against Apoptosis by Promoting Autophagy in the Oxygen Glucose Deprivation/Reperfusion-Induced Injury

SIRT1 Protects Against Apoptosis by Promoting Autophagy in the Oxygen Glucose Deprivation/Reperfusion-Induced Injury

Blog Article

Silent information regulator 1 (SIRT1) contributes to cellular regulation.Previous studies have reported SIRT1 to be abnormally expressed in the ischemic penumbra of cerebral ischemia/reperfusion (I/R) injury rat model.We investigated the effect of SIRT1 on oxygen and glucose deprivation/reperfusion (OGD/R) cell injury.Over-expressed Construction of local and regional identity or silenced SIRT1 pheochromocytoma 12 (PC12) cells were exposed to an in-vitro OGD/R injury.Western blot, TUNEL staining and immunofluorescence analyses were performed to assess apoptosis and autophagy.

We found autophagy and apoptosis to be up-regulated and down-regulated, respectively, following the over-expression of SIRT1 in the OGD/R-induced PC12 cells.We also found the silencing of SIRT1 to culminate Desenvolvimento do pensamento crítico por meio do estudo de lógica argumentativa na alfabetização científica in the down-regulation and up-regulation of autophagy and apoptosis, respectively.On the basis of our results, we surmise that SIRT1 can promote autophagy and inhibit apoptosis in-vitro, and thus exhibit potential neuroprotection against OGD/R-induced injury.This could facilitate in the development of therapeutic approaches for cerebral I/R injury.